Agenda • Background • Next Generation Sequencing • Sequence Assembly • Sequence Alignment • Traditional Alignment Algorithms • Next Generation Alignment Algorithms • Conclusion Andy Nagar 2 Background • Earlier sequencing methods were based on Sanger sequencing, which goes back to the 1970s. • Sequencing was slow, bases were read ...
Work flow of conventional versus second- generation sequencing (a) With high-throughput shotgun Sanger sequencing, genomic DNA is fragmented, then cloned to a plasmid vector and used to transform E. coli. For each sequencing reaction, a single bacterial colony is picked and plasmid DNA isolated. Each cycle sequencing reaction takes place ...
Content Forensic Science and DNA fingerprinting Invention of DNA fingerprinting Preparation of DNA Fingerprint Steps of DNA fingerprinting Types of Analysis RFLP, PCR, STR Analysis, AFLP Analysis Applications DNA Fingerprinting and Forensic Science Forensic science Intersection of Law and Science Historic examples of collecting evidences: 18th Century – Photography ...
Introduction DNA sequencing Single molecule Multiple molecules Some disadvantages for sequencing multiple mol ecules Expensive and time-consuming sample preparation Difficult to discover repeated sequences A single-molecule physics-based sequencing meth od is introduced Quantum sequencing Quantum sequencing(1/2) Ref : Going Solid-State,Elizabeth Pennisi, Science 4 May 2012: Vol. 336 no. 6081 ...
Sanger (dideoxy) DNA sequencing procedure overview •The reaction is a DNA synthesis reaction which involves: •a high concentration of regular deoxynucleotides •a low concentration of dye-labeled dideoxynucleotides •Each of the four dideoxynucleotides (A,T,C,G) is labeled with a different color dye. •After the ...
Overview Southern-blot DNA Digestion Based hybridization COBRA MSP Bisulfite Sequencing Bisulfite Based Real-time MSP MethyLight MassARRAY Pyrosequencing Southern Blot Hybridization 1978 -One of the older techniques for sequencing (1978), not as widely used any more due to the development of PCR and other cheap/fast sequencing techniques, not outdated but just ...
INTRODUCTION The term DNA sequencing refers to sequencing methods for determining the order of the nucleotide bases – adenine, guanine, cytosine, and thymine - in a molecule of DNA. Knowledge of DNA sequences has become indispensable for basic biological research, other research branches utilizing DNA sequencing, and in numerous applied ...
DNA fragment sequencing In order to establish exactly what changes have occurred in the gene under study, it will be necessary determine the DNA sequence of that gene. This is now a routine procedure in molecular biology laboratories, extending not just to individual genes or fragments but to the complete ...
Our Mtb DNA on the Tapestation MinION fastq file 2 records – 576 bp; 1316 bp Illumina fastq file all records ~100 bp There are 3 main approaches to sequencing Market Approach / USP Read Achievements Ideal for: dominance length st 1 Generation Applied Sequencing by 1 kb Most primary ...
Instructors - Bio-Rad Curriculum and Training Specialists Sherri Andrews, Ph.D., Eastern US sherri_andrews@bio-rad.com Damon Tighe, Western US damon_tighe@bio-rad.com Leigh Brown, M.A., Central US leigh_brown@bio-rad.com 2 Biotechnology Explorer™ | explorer.bio-rad.com Workshop Timeline Introduction Background on DNA Extraction Extract Genomic DNA from Cheek Cells Prepare ...
Kickoff: February 21, 2020 DNA Assays for Determining Honey Origins Dr. Richard C. Willson, University of Houston (PI) Dr. Aniko Sabo, Baylor College of Medicine (Expert in Bioinformatics) Dr. Katerina Kourentzi, University of Houston (Expert in Assay development) Other personnel: Dimple Chavan, University of Houston (Graduate student) Suman Nandy, University ...
What is gel electrophoresis? - - - - - - - wells DNA (-) ++++++ + Loading pcr Buffer products STAIN DNA Sequencing DNA Sequencing: Basic Principle Sequence of base pairs?? A G G T C A A G C T A T C polymerase ...
DNA sequencing How we obtain the sequence of nucleotides of a species ACGTGACTGAGGACCGTG CGACTGAGACTGACTGGGT CTAGCTAGACTACGTTTTA TATATATATACGTCGTCGT ACTGATGACTAGATTACAG ACTGATTTAGATACCTGAC TGATTTTAAAAAAATATT Which representative of the species? Which human? Answer one: Answer two: it doesn’t matter Polymorphism rate: number of letter changes between two different members of a species Humans: ~1/1 ...
PENDAHULUAN Bioteknologi adalah penggunaan biokimia, mikrobiologi, dan rekayasa genetika secara terpadu, untuk menghasilkan barang atau lainnya bagi kepentingan manusia Rekayasa genetika merupakan aplikasi genetik dengan mentransplantasi gen dari satu organisme ke organisme lain. Bioteknologi sangat berkaitan dengan bidang ilmu : biologi molekuler, mikrobiologi, biokimia, genetika kimia, rekayasa teknik, bioinformatika dan ...
DNA sequencing ACGTGACTGAGGACCGTG CGACTGAGACTGACTGGGT CTAGCTAGACTACGTTTTA TATATATATACGTCGTCGT ACTGATGACTAGATTACAG ACTGATTTAGATACCTGAC TGATTTTAAAAAAATATT DNA sequencing Determination of nucleotide sequence Two similar methods: 1. Maxam and Gilbert method 2. Sanger method They depend on the production of a mixture of oligonucleotides labeled either radioactively or fluorescein, with one common end and differing in length ...
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