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picture1_Dna Sequencing Slideshare 66839 | Dna Sequencing


picture2_Dna Sequencing Slideshare 66839 | Dna Sequencing picture3_Dna Sequencing Slideshare 66839 | Dna Sequencing

 142x       Filetype PPT       File size 1.25 MB       Source: faperta.ugm.ac.id


File: Dna Sequencing Slideshare 66839 | Dna Sequencing
DNA sequencing …ACGTGACTGAGGACCGTG CGACTGAGACTGACTGGGT CTAGCTAGACTACGTTTTA TATATATATACGTCGTCGT ACTGATGACTAGATTACAG ACTGATTTAGATACCTGAC TGATTTTAAAAAAATATT… DNA sequencing Determination of nucleotide sequence Two similar methods: 1. Maxam and Gilbert method 2. Sanger method They depend on ...

icon picture PPT Filetype Power Point PPT | Posted on 28 Aug 2022 | 2 years ago

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...Dna sequencing acgtgactgaggaccgtg cgactgagactgactgggt ctagctagactacgtttta tatatatatacgtcgtcgt actgatgactagattacag actgatttagatacctgac tgattttaaaaaaatatt determination of nucleotide sequence two similar methods maxam and gilbert method sanger they depend on the production a mixture oligonucleotides labeled either radioactively or fluorescein with one common end differing in length by single at other this is separated high resolution electrophoresis polyacrilamide gels position bands determined stranded fragment to be sequenced treatment alkaline phosphatase remove phosphate it then followed reaction p atp presence polynucleotide kinase which attaches terminal divided into four aliquots each treated reagent modifies specific base aliquot dimethyl sulphate methylates guanine residue b formic acid adenine residues c hydrazine thymine cytosine d mol l nacl makes for are incubated piperidine cleaves sugar backbone next that has been modified frederick discovered chain termination nobel prize...
								

							

						
	
												
				
					
					
					
			
					
				
				
		
	

	
		





	

	
				

						

						
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										Category:  Uncategorized / Uncategorized 
										
Posted on: 28 Aug 2022 | 2 years ago
										
										
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Filetype: PPT
										
File size: 1.25 MB
										
Views: 142x
										
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										Wan learn
											

												Wan learn