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picture1_Dna Sequencing Slideshare 66904 | Sanger Sequencing Slides Edited


picture2_Dna Sequencing Slideshare 66904 | Sanger Sequencing Slides Edited picture3_Dna Sequencing Slideshare 66904 | Sanger Sequencing Slides Edited

 156x       Filetype PPTX       File size 0.16 MB       Source: legacy.genetics-gsa.org


File: Dna Sequencing Slideshare 66904 | Sanger Sequencing Slides Edited
Sanger (dideoxy) DNA sequencing procedure overview •The reaction is a DNA synthesis reaction which involves: •a high concentration of regular deoxynucleotides •a low concentration of dye-labeled dideoxynucleotides ...

icon picture PPTX Filetype Power Point PPTX | Posted on 28 Aug 2022 | 2 years ago
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...Sanger dideoxy dna sequencing procedure overview the reaction is a synthesis which involves high concentration of regular deoxynucleotides low dye labeled dideoxynucleotides each four t c g with different color after produced imaged during electrophoresis to detect colors associated band sizes materials for model blocks represent nucleotides paper represents template dntps we will be black determining sequence maroon brown white ddntps orange classroom microtube and student yellow lt blue polymerase green first steps in simulation make two copies primer att composed this would modeled chain denature by cutting tearing separate strands reality accomplished heating sample anneal strand modeling roll die if rolled incorporate appropriate deoxynucleotide at next position dideoxynucleotide that terminated repeat above until incorporated synthesize one it then those second using another carefully keep your newly synthesized chains all complete products electrophoresed...
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