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picture1_Dna Sequencing Slideshare 68184 | Chipseq


picture2_Dna Sequencing Slideshare 68184 | Chipseq picture3_Dna Sequencing Slideshare 68184 | Chipseq

 140x       Filetype PPTX       File size 1.78 MB       Source: www.cs.utsa.edu


File: Dna Sequencing Slideshare 68184 | Chipseq
Work flow of conventional versus second- generation sequencing (a) With high-throughput shotgun Sanger sequencing, genomic DNA is fragmented, then cloned to a plasmid vector and used to transform E. coli ...

icon picture PPTX Filetype Power Point PPTX | Posted on 28 Aug 2022 | 2 years ago

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...Work flow of conventional versus second generation sequencing a with high throughput shotgun sanger genomic dna is fragmented then cloned to plasmid vector and used transform e coli for each reaction single bacterial colony picked isolated cycle takes place within microliter scale volume generating ladder ddntp terminated dye labeled products which are subjected resolution electrophoretic separation one or capillaries in run instrument as fluorescently fragments discrete sizes pass detector the four channel emission spectrum generate trace b cyclic array methods common adaptors ligated several protocols that results an millions spatially immobilized pcr colonies polonies polony consists many copies library fragment all tethered planar reagent g primer hybridization enzymatic extension reactions can be applied manipulate features parallel similarly imaging based detection fluorescent labels incorporated acquire data on successive iterations interrogation build up contiguous read feature...
								

							

						
	
												
				
					
					
					
			
					
				
				
		
	

	
		





	

	
				

						

						
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										Category:  Uncategorized / Uncategorized 
										
Posted on: 28 Aug 2022 | 2 years ago
										
										
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												Wan learn