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picture1_Dna Sequencing Slideshare 66998 | Nextgenerationsequencing


picture2_Dna Sequencing Slideshare 66998 | Nextgenerationsequencing picture3_Dna Sequencing Slideshare 66998 | Nextgenerationsequencing

 213x       Filetype PPTX       File size 1.82 MB       Source: s2.smu.edu


File: Dna Sequencing Slideshare 66998 | Nextgenerationsequencing
Agenda • Background • Next Generation Sequencing • Sequence Assembly • Sequence Alignment • Traditional Alignment Algorithms • Next Generation Alignment Algorithms • Conclusion Andy Nagar 2 Background • Earlier ...

icon picture PPTX Filetype Power Point PPTX | Posted on 28 Aug 2022 | 2 years ago
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...Agenda background next generation sequencing sequence assembly alignment traditional algorithms conclusion andy nagar earlier methods were based on sanger which goes back to the s was slow bases read one at a time separation is done by electrophoresis readout fluorescent tags source complete second genome projects such as human project need for faster and high throughput technologies various implementations of cyclic array idea an dna features continuous process enzymatic imaging data collection growth in gen doubles every month workflow fragmented adaptors ligated fragments several possible protocols yield pcr colonies enyzmatic extension with fluorescently tagged nucleotides...
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