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...Introduction the term dna sequencing refers to methods for determining order of nucleotide bases adenine guanine cytosine and thymine in a molecule knowledge sequences has become indispensable basic biological research other branches utilizing numerous applied elds such as diagnostic biotechnology forensic biology systematics principle purification fragment that be sequenced labeled with radioactive material chemical treatment generates breaks at specific nitrogenous thus series labelled fragments is generated four reactions are arranged side by gel electrophoresis size separation visualize x ray autoradiography exposed film yielding dark bands each corresponding radiolabelled from which sequence may inferred applications its study we can understand function responsible any disease help comparative detect mutation fingerprinting knowing whole genome human project get completed forensics been science identify particular individual because every unique his her it particularly need crimin...