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...The term tissue culture may be defined as process of in vitro explants nutrient medium under aseptic conditions it includes cell organ and suspension etc german botanist gottlieb haberlandt was regarded father basis is totipotency first attempted to plant tissues he started his work used cells from palisade leaves pith epidermis epidermal hairs various plants for media containing knop s solution asparagine peptone sucrose main requirements are laboratory organisation i a room preparation sterilization storage ii facilities washing lab wares iii space iv rooms or incubators where temperature humidity light can maintained v observation data collection area...