plant tissue culture plant tissue culture is used to create clones this is useful to create pathogen free species create crop species that have identical traits e g height save ...
Filetype Power Point PPT | Posted on 03 Sep 2022 | 2 years ago
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...Plant tissue culture is used to create clones this useful pathogen free species crop that have identical traits e g height save endangered somatic fusion protoplast hybridisation new varieties then grow the cells from two different plants are selected cell wall removed using cellulase and pectinase protoplasts fused either polyethylene glycol electrical shock grown source of small pieces taken a these called explants long life span suitable container anything sterile entire procedure must be carried out under aseptic conditions cont growth medium simple can liquid or solid containing carbon minerals salts water vitamins contains regulators cause differentiation cytokinins promote shoot development auxins root events in explant sterilised it transferred media mass undifferentiated callus forms occurs due auxin cytokinin concentrations being balanced production acclimatised greenhouses...