Plant Tissue Culture and their techniques What is plant tissue culture? Plant tissue culture is a technique of growing plant cells, tissues, organs, seeds or other plant parts in a sterile environment on a nutrient medium Fundamental principles: PTC depends upon 1. Totipotency :- It is the ability of plant cells to regenerate into a whole plant 2. Plasticity :- It is the ability of plants to alter their metabolism, growth and development to best suit their environment Expla nt Plant tissue cultures are generally initiated from multicellular tissue fragments, called explants, obtained from living plants. Explants may originate from ...
Setting Up a Tissue Culture Lab Any laboratory, in which tissue culture techniques are performed, regardless of the specific purpose, must contain a number of basic facilities. These usually include the following: • A general washing area • A media preparation, sterilization, and storage area • An aseptic transfer area • Environmentally controlled incubators or culture rooms • An observation/data collection area. Nutrient media: The type and composition of culture media very strongly govern the growth and morphogenesis of plant tissues. The choice of tissue culture medium largely depends upon the species to be cultured. For e.g. some species ...
Biotechnology in Agriculture There are many important applications of biotechnology that have made a trem endous impact on agricultural productivit y. Conventional plant breeding Tissue culture & micropropagation Molecular breeding or marker assisted selection Genetic engineering & GM crops The ‘Omics’ - Genomics, Proteomics, Metabolomics Plant disease diagnostics Microbial fermentation. Biotechnology in Agriculture GE of animals GE of animals GE of plants GE of plants GE to improve GE to develop GE to improve GE to develop microorganisms animal vaccines microorganisms animal vaccines Recombinant Recombinant GE of biocontrol DNA for disease GE of biocontrol DNA for disease ...
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