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...Journal of tissue culture and bioengineering review article chimdessa e cult bio bioeng doi composition preparation plant medium emiru oromia agricultural research institute ethiopia corresponding author batu citation received date december accepted published abstract tissues organs are grown in vitro on artificial media which supply the nutrients necessary for growth a is complete mixture regulators basic nutrient requirements cultured cells very similar to those whole plants clue developing seems have initially come from nutritional growing soil later solutions used when mineral salts dissolved water they undergo dissociation ionization active factor ions different types rather than compounds one type ion may be contributed by more salt therefore meaningful comparison between two can made basis total concentrations them most commonly formulation murashige skoog ms or linsmaier ls widely compositions especially procedures where regeneration objective introduction as such no single sug...