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...Chapter plant tissue culture media abobkar i m saad and ahmed elshahed additional information is available at the end of http dx doi org introduction optimal growth morphogenesis tissues may vary for different plants according to their nutritional requirements moreover from parts also have satisfactory were first developed nutrient solutions used culturing whole e g root medium white callus gautheret s was based on uspenski uspenska algae knop salt solution basic that are frequently include murashige skoog ms linsmaier ls gamborg b nitsch nn composition should generally contain some or all following components macronutrients micronutrients vitamins amino acids nitrogen supplements source carbon undefined organic regulators solidifying agents international association physiology elements in concentrations greater than mm l defined as macroelements those required less microelements it be considered optimum concentration each achieving maximum rates varies among species essential cell bes...