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Table of Content How it Looks Introduction Principle of HPLC Instrumentation pf HPLC Types of HPLC Comparison of HPLC with different Chromatography Why We use HPLC Applications Advantages Limitation References How it Looks 3 Introduction High performance liquid chromatography or ...
• Objective: Simple high performance liquid chromatographic (HPLC) and ultraviolet (UV) methods were newly developed and validated for the quantitative estimation of Montelukast (ML) in pharmaceutical dosage form. • Material and Methods: HPLC was carried out by reverse phase-HPLC technique ...
High-Performance Liquid Chromatography (HPLC) Systems Image reference: http://www.pharmatips.in/Articles/Pharmaceutical-Equipment/HPLC-Operation- Calibration.aspx Introduction Of HPLC • HPLC stands for “High-performance liquid chromatography”(sometimes referred to as High- pressure liquid chromatography). • Chromatography : physical method in which separation of ...
Q:1: The analytical technique in which optics (Photometry) is NOT used in any form: a. Atomic Absorption b. HPLC c. POCT for glucose d. Radioimmunoassay e. Real Time PCR Best answer: d. Radioimmunoassay Optical Techniques used in Various Instruments Atomic ...
Zwitterionic Stationary Phase in HPLC Outline • Introduction • Theory • Advantages and Disadvantages • Some Applications • Conclusions • References • Questions Zwitterionic Stationary Phase in HPLC Introduction • Knox and Jurand (1981) o Separation of nucleotides on a ...
THEORY OF HPLC 20% of compds. Can be analyzed by GC. Because of non-volatility & thermal instability. HPLC is not limited by sample volatility, Thermal stability. It can separate macromolecules, ionic sp., labile natural products, polymeric mat., high mol ...
• High Performance Liquid Chromatography (HPLC) is a form of column chromatography that pumps a sample mixture or analyt in a solvent (known as the mobile phase) at high pressure through a column with chromatographic packing material (stationary phase). &bull ...
Outline History and Background Theory Advantages Disadvantages Applications Conclusions History First reported as high-pressure gas chromatography (HPGC) before HPLC in 1962. 1966 first use of supercritical CO as mobile phase 2 Used a UV absorption detector with a quartz cell ...
LC-MS? LIQUID CHROMATOGRAPHY MASS SPECTROMETRY • Separates • Component components identification is superior • Identification from retention time is • But, interference difficult from other ions Ardrey, R. E. Liquid Chromatography Mass spectrometry: an introduction, 2 Wiley, West Sussex, England ...
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Determination of metformin in biological fluids (human plasma, urine, etc) Challenge to conventional chromatographic methods Metformin • Oral antidiabetic drug • Small molecule with high polarity (impossible to maintain by RP columns) • pKa1=2.8 and pKa2=11.5 Determination of ...
Determination of metformin in biological fluids (human plasma, urine, etc) Challenge to conventional chromatographic methods Metformin • Oral antidiabetic drug • Small molecule with high polarity (impossible to maintain by RP columns) • pKa1=2.8 and pKa2=11.5 Determination of ...
PENGERTIAN • Detektor Indeks Bias detektor indeks bias merupakan detektor yang juga luas penggunaannya setelah detektor ultraviolet. • Dasarnya pengukuran perbedaan indeks bias fase gerak murni dengan indeks bias fase gerak yang berisi komponen sampel, sehingga dapat dianggap sebagai detektor ...
Principles Principles A gaseous mobile phase flows under pressure through a heated tube either coated with a liquid stationary phase or packed with liquid stationary phase coated onto a solid support. The analyte is loaded onto the head of the ...
Standard solution of Compounds of Interest and Required Components • Make a 5 mg/mL solution of compounds • Dilute 5 mg/mL compound solution with water to yield a 50 ug/mL stock solution, experiment requires very low organic content • Required ...
Introduction • Chromatography was discovered by Russian botanist Mikhail Semyonovich Tsvett, who separated plant pigments using calcium carbonate columns (1901) • Martin and Synge (NP 1952) established many of the basic techniques in partition chromatography i.e., paper chromatography, gas ...
Introduction • Chiral stationary phase are used in gas-liquid chromatography (GC/GLC) and liquid-solid chromatography (HPLC) • Chiral GC columns are frequently used in pharmaceutical research (i.e., enantiomeric purity of drugs), quality control of nature products, forensics, etc. • Commonly ...
Announcements • Set 1 Lab Reports – hand back • Set 2 Lab Reports – Due 4/10 • Final Exam – April 15th • Future Mondays (after 4/15) – Set 3 Presentation – 4/29 – 4/22 and other Mondays &bull ...
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