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picture1_Hplc Slideshare 67164 | Optimization Experiment Video Notes Update


picture2_Hplc Slideshare 67164 | Optimization Experiment Video Notes Update picture3_Hplc Slideshare 67164 | Optimization Experiment Video Notes Update

 136x       Filetype PPTX       File size 0.12 MB       Source: www.agilent.com


File: Hplc Slideshare 67164 | Optimization Experiment Video Notes Update
Standard solution of Compounds of Interest and Required Components • Make a 5 mg/mL solution of compounds • Dilute 5 mg/mL compound solution with water to yield a 50 ug/mL ...

icon picture PPTX Filetype Power Point PPTX | Posted on 28 Aug 2022 | 2 years ago
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...Standard solution of compounds interest and required components make a mg ml dilute compound with water to yield ug stock experiment requires very low organic content solvents methanol di collection tubes or larger tube volume pipettes spe port manifold material agilent bond elut c p n will need cartridges accomplish both the high ph hplc for analysis research use only not in diagnostic procedures generic procedure optimization place on one cartridge per then repeat experiments shown below is if used instead change all volumes condition load under every prior addition wash individual solutions collect each from analyze by general overall steps determine recovery perform equilibrate twenty as method standards real samples prepared no final elution step dry down reconstitute mobile phase add istd needed necessary data calculate recoveries sample information be determined extraction optimized meoh h ho elute employing systematic development approach offers simple highly selective when com...
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