Hplc Slideshare 68162 | Chem 14cl Lecture 7a Chromatography Cc Tlc

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File: Hplc Slideshare 68162 | Chem 14cl Lecture 7a Chromatography Cc Tlc

introduction chromatography was discovered by russian botanist mikhail semyonovich tsvett who separated plant pigments using calcium carbonate columns 1901 martin and synge np 1952 established many of the basic techniques ...

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...Introduction chromatography was discovered by russian botanist mikhail semyonovich tsvett who separated plant pigments using calcium carbonate columns martin and synge np established many of the basic techniques in partition i e paper gas hplc etc is used separation purification smaller quantities whereas distillation recrystallization for large scale separations purifications sample completely adsorbed on stationary phase before mobile solvent moves across strength interaction compound with solubility as well eluting power will dictate degree migration quality compounds a mixture based different affinities thus each has coefficient between these two phases higher affinity towards lower less migrate resulting later elution from cc or r value tlc f commonly are silica alumina cellulose o al considered polar because presence hydroxyl groups surface they can be modified attaching non to functions long hydrocarbon chains c coated plates primarily organic labs most analyzed lab weakly due c...

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Posted on: 28 Aug 2022 | 3 years ago
Tag: download pptx, chem, 14cl, lecture, chromatography,
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