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File: Dna Sequencing Methods Pdf 86050 | 100004147
1 DNA Sequencing (NOTES) DNA sequencing is the process of determining the exact order of nucleotides within a DNA molecule. This method is used to determine the order of the ...

icon picture PDF Filetype PDF | Posted on 14 Sep 2022 | 2 years ago

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...Dna sequencing notes is the process of determining exact order nucleotides within a molecule this method used to determine four bases adenine guanine g cytosine cy and thymine t in strand advent rapid methods has greatly accelerated biological medical research nitrogenous structure been feasible because following developments availability restriction endonucleases development highly sensitive gel electrophoresis technique which can separate fragments differing by only one nucleotide large quantities individual due gene cloning pcr techniques reliable easy there are two basic chemical explored maxam gilbert enzymatic sanger allan walter frederick procedure published based on modification subsequent cleavage at specific allows purified samples double stranded be without further requires radioactive labelling end or followed purification fragment sequenced steps kinase reaction using p cut with enzyme resulting unequal denature single increasing temperature cleave positions reactions for ...
								

							

						
	
												
				
					
					
					
			
					
				
				
		
	

	
		





	

	
				

						

						
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Posted on: 14 Sep 2022 | 2 years ago
										
										
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