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...The principles of dna sequencing process determining order nucleotide bases along a strand is called in twenty four years after discovery structure two separate methods for were developed chain termination method and chemical degradation both equally popular to begin with but many reasons more commonly used today this based on principle that single stranded molecules differ length by just can be separated from one another using polyacrylamide gel electrophoresis described earlier sequenced template first prepared as next short oligonucleotide annealed or joined same position each acts primer synthesis new will complimentary technique requires specific reactions g c t performed identical samples require addition all components necessary synthesize label including tagged mildly radioactive molecule light emitting polymerase an enzyme drives deoxynucleotides dideoxynucleotide either ddg dda ddc ddt deoxynucleotide added growing complementary sequence moves continues add base reaction unti...