A Practical Guide to SPM Scanning Probe Microscopy A Practical Guide to SPM TABLE OF CONTENTS 4 I. INTRODUCTION 4 II. HOW AN SPM WORKS 4 The Probe 5 The Scanner 5 Scanning: Setpoint, Detector Signal, and Error Signal 6 The SPM Image 6 The Z Feedback Loop 6 Scanning Tunneling Microscopy (STM) 7 Atomic Force Microscopy (AFM) 8 III. NEAR-FIELD SCANNING OPTICAL MICROSCOPY (NSOM) ...
Indian Institute of Technology (Indian School of Mines) Indian Institute of Technology (Indian School of Mines) Dhanbad, Jharkhand – 826004 Dhanbad, Jharkhand 826004 Central Research Facility http://iitism.ac.in/index.php/Research_Consultancy/central_research_facility Scanning Probe Microscopy With STM, AFM, LFM, MFM, EFM, Nanoindentation Scanning Probe Microscope Laboratory has two SPM systems (Dimension Icon and MultiMode 8) with one controller (Nanoscope V) from Bruker Corporation. Hence, only one system ...
2 Scanning Probe Microscopes (SPMs) • Monitor the interactions between a probe and a sample surface • What we “see” is really an image • Two types of microscopy we will look at: – Scanning Tunneling Microscope (STM) – Atomic Force Microscope (AFM) 3 Scanning Tunneling Microscopes (STMs) • Monitors the electron tunneling current between a probe and a sample Scanning Probe surface &bull ...
Biotechniques microscopy Dr. S.D. SARASWATHY Assistant Professor Department of Biomedical Science Bharathidasan University Tiruchirappalli KEY CONCEPTS •Introduction •Basic Principle of Light microscopy •Instrumentation •Types and Applications MICROSCOPY-INTRODUCTION • Microscopy is defined as the use of a microscope to magnify and study the small objects that are too small to be visualized with the naked eye. • Basically 3 main types: ...
MICROSCOPYRESEARCHANDTECHNIQUE70:2633(2007) CombiningOpticalTweezersandScanningProbe MicroscopytoStudyDNA–ProteinInteractions * JURGENH.G.HUISSTEDE,VINODSUBRAMANIAM,ANDMARTINL.BENNINK BiophysicalEngineeringGroupandMESAþInstituteforNanotechnology,FacultyofScienceandTechnology, University of Twente, AE Enschede 7500, The Netherlands KEYWORDS optical tweezers; scanning probe microscopy; DNAprotein interactions; dig- oxygenin; single molecule force spectroscopy ABSTRACT Wepresenttherst results obtained with a new instrument designed and built to study DNAprotein interactions at the single molecule level. This microscope combines optical tweezers with scanning probe microscopy ...
Module -5 _ Unit-2 Scanning Probe Microscopy (STM, AFM) Scanning probe microscopy was discovered in early 1980s, when it splendid the world by producing the first real-space images of surfaces with an unprecedented resolution of atomic-scale. Presently, SPM is one of the most employed technique in various disciplines which include fundamental surface science, routine examinations of the surface roughness, and three-dimensional imaging (from the atoms ...
Lecture 4 Scanning Probe Microscopy (SPM) • General components of SPM; • Tip --- the probe; • Cantilever --- the indicator of the tip; • Tip-sample interaction --- the feedback system; • Scanner --- piezoelectric movement at x,y,z; • Measurement artifacts: vibration must be isolated. Generation of SPM image X-Y raster scanning; Z-modulation (height) by feedback system. Basic components of SPM: tip, cantilever ...
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