File: Autoradiography Pdf 90255 | 577 Item Download 2022-09-16 00-22-03
A METHOD FOR INTRACELLULAR AUTORADIOGRAPHY IN THE ELECTRON MICROSCOPE M. H. SILK, Ph.D., A. O. HAWTREY, Ph.D., I. M. SPENCE, and J. H. S. GEAR From the Poliomyelitis ...
Filetype PDF | Posted on 16 Sep 2022 | 2 years ago
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...A method for intracellular autoradiography in the electron microscope m h silk ph d o hawtrey i spence and j s gear from poliomyelitis research foundation johannesburg south africa dr present address is fishing industry institute university of cape town rondebosch abstract technic described high resolution cultures llc mk monkey kidney cells were incubated hours medium containing curie per ml thymidine after labeling fixed with osmium tetroxide embedded methacrylate ultrathin sections labeled tissue taken up on formvar coated carbon stabilized grids to layer silver metal was then evaporated onto exposed bromine vapor minutes under red light allowed dry minute covered thin film cent aqueous gelatin applied by means fine wire loop lowered over grid supported glass peg autoradiographic exposure stored days proof container at c calcium chloride desiccant development carried out promicrol may baker england diluted water followed wash fixa tion sodium thiosulphate removal immersion autoradio...