AUTORADIOGRAPHY DR.RUCHIKA YADU INTRODUCTION • Autoradiography is the bio-analytical technique used to visualize the distribution of radioactive labeled substance with radioisotope in a biological sample. • It is a method by which a radioactive material can be localized within a particular tissue, cell, cell organelles or even biomolecules. • It is a very sensitive technique and is being used in a wide variety of biological experiments. • Autoradiography, although used to locate the radioactive substances, it can also be ...
Data Sheet Autoradiography Protocol Contents IN VITRO AUTORADIOGRAPHY ....................................................................................................... 2 Sectioning .................................................................................................................................... 2 Incubation in radiotracer ............................................................................................................ 2 Phosphorimaging......................................................................................................................... 2 Data analysis ................................................................................................................................ 2 EX VIVO AUTORADIOGRAPHY ......................................................................................................... 2 Sectioning .................................................................................................................................... 2 Phosphorimaging......................................................................................................................... 3 Data analysis ................................................................................................................................ 3 EX VIVO RECEPTOR OCCUPANCY ................................................................................................... 3 Sectioning .................................................................................................................................... 3 Incubation in radiotracer ............................................................................................................ 3 Phosphorimaging......................................................................................................................... 3 Data analysis ................................................................................................................................ 3 info@giffordbioscience.com www.giffordbioscience.com Data Sheet Autoradiography Protocol IN VITRO AUTORADIOGRAPHY Sectioning Frozen brains from drug-treated animals are trimmed with a razor blade and mounted in a cryostat chuck. Tissue sections are ...
New developments of autoradiography technique to improve alpha and beta measurements for decommissioning facilities P Fichet (1), C Mougel (1), I Desnoyers (2), P Sardini (3), H Word (4) (1) Den – Service d’Etudes Analytiques et de Reactivite des Surfaces (SEARS), CEA (Alternative Energies and Atomic Energy Commission, France), Universite Paris- Saclay, F-91191, Gif sur Yvette, France (2) Geovariances 49bis avenue Franklin Roosevelt - 77215 Avon cedex – France (3) IC2MP- HydrASA / UMR 7285 CNRS, 86073 Poitiers cedex ...
r t i e c m s o & i B B f i o o s l t Khan, J Biom Biostat 2017, 8:4 a a n t ur is DOI: 10.4172/2155-6180.1000361 oJ scitJournal of Biometrics & Biostatistics ISSN: 2155-6180 Research Article Open Access Review Article Autoradiography: Detection and Analysis of Radioactive Entities Nida Tabassum Khan* Department of Biotechnology, Faculty of Life Sciences and Informatics, Balochistan University of Information Technology Engineering and Management Sciences, (BUITEMS) ...
A METHOD FOR INTRACELLULAR AUTORADIOGRAPHY IN THE ELECTRON MICROSCOPE M. H. SILK, Ph.D., A. O. HAWTREY, Ph.D., I. M. SPENCE, and J. H. S. GEAR From the Poliomyelitis Research Foundation, Johannesburg, South Africa. Dr. Silk's present address is Fishing Industry Research Institute, University of Cape Town, Rondebosch, Cape Town, South Africa ABSTRACT A technic is described for high resolution intracellular autoradiography in the electron microscope. Cultures of LLC-MK2 monkey kidney cells were incubated for 72 hours in ...
“Methods in Histology” Objectives: Understand the uses of the most important types of light microscopes Understand the basic operation and uses of electron microscopes Understand “resolution” and some basic units of size Understand basic steps in tissue preparation for light and electron microscopy Know major staining methods used in histology and what cell components are visualized with the different stains Understand basic principles and uses for other specific histological techniques, including enzyme histochemistry, immunohistochemistry, in situ hybridization, and ...
- Y77) - STUK-YTO-TR 99 Detection and analysis of radioactive particles using autoradiography Report on task FIN A845 on the Finnish support programme to IAEA safeguards R. Pollanen, A. Kansanaho, H. Toivonen FEBRUARY 1996 : -''':'''';'. "'-". ','. -V ^; ''.-'... ., ..--:,.- ;* ':.' 1 '":' .*.-„' ' .* '":.".'.'' ...
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