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...Data sheet autoradiography protocol contents in vitro sectioning incubation radiotracer phosphorimaging analysis ex vivo receptor occupancy info giffordbioscience com www frozen brains from drug treated animals are trimmed with a razor blade and mounted cryostat chuck tissue sections cut at thickness of m using bright otf thaw onto superfrost slides three consecutive placed on each slide total brain region stored dessicated o c warmed to room temperature whilst still the box then pre buffer mm tris mgcl edta protease inhibitor cocktail ph for min gentle agitation removed horizontally humidified ml radioligand assay layered over incubated solution periodic is rapidly aspirated off immediately ice cold wash washes following final dipped briefly distilled water dried under stream warm air after drying multipurpose i or tritium sensitive h phosphor screen together autoradiographic standards exposed days scanned phosphorimager cyclone storage system regions interest rois drawn section bindi...