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picture1_Northern Blotting Pdf 90143 | P 043 Northern Blotting


picture2_Northern Blotting Pdf 90143 | P 043 Northern Blotting picture3_Northern Blotting Pdf 90143 | P 043 Northern Blotting

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File: Northern Blotting Pdf 90143 | P 043 Northern Blotting
1 TMM,4-06 Northern Blotting (acc. to Maniatis) For work with RNA: Be aware of RNases! Use gloves, wash gloved hands with water, dry. Always use freshly autoclaved not yet ...

icon picture PDF Filetype PDF | Posted on 15 Sep 2022 | 2 years ago

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...Tmm northern blotting acc to maniatis for work with rna be aware of rnases use gloves wash gloved hands water dry always freshly autoclaved not yet opened pipet tips eppis treat pipets rnase erase spray preparation gel and buffers depc inhibits activity in millipore let stand on autoclave instead may used x running buffer m mops ph mm sodium acetate edta dissolve g acetetate ml sterile adjust add l turns yellow store at rt alufoil special chambers well comb chamber or other reagent inactivation as requested then wipe medium size requires melt appropriate amount agarose rnas from kb is ok cool c formaldehyde mg ethidium bromide the hood if gets cold it might get solid do reheat allow set least min denature so that no secondary structures can form runs according its actual length sample precipitate up total lane has stored a just spin also marker same way once etoh completely pellet rid all salt otherwise bands run uneven smilies typically either polya resuspend when dissolved formamide ...
								

							

						
	
												
				
					
					
					
			
					
				
				
		
	

	
		





	

	
				

						

						
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										Category:  Uncategorized / Uncategorized 
										
Posted on: 15 Sep 2022 | 2 years ago
										
										
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Filetype: PDF
										
File size: 0.11 MB
										
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										Wan learn
											

												Wan learn