Molecular Blotting techniques Prof. Dr. ArttatranaPal Department ofZoology School of LifeSciences MahatmaGandhiCentral University BiharIndia What is blotting? Blots are techniques for transferrin DNA, RNA and g proteins into a carrier so they can be separated, and often follows the use of a gel electrophoresis Types of blotting Techniques Southern Blot: It is used to detect DNA. Northern Blot: It is used to detect RNA. Western ...
583 zyxwvutsrqponmlkjihgfedcbaZYXWVUTSRQPONMLKJIHGFEDCBA Proceedings zyxwvutsrqponmlkjihgfedcbaZYXWVUTSRQPONMLKJIHGFEDCBAof the zyxwvutsrqponmlkjihgfedcbaZYXWVUTSRQPONMLKJIHGFEDCBANutrition Society zyxwvutsrqponmlkjihgfedcbaZYXWVUTSRQPONMLKJIHGFEDCBA(1996), 55, 583-589 Northern blotting BY PAUL TRAYHURN Division of Biochemical Sciences, Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB INTRODUCTION: WHY MEASURE AN mRNA? Northern blotting is one of the key techniques in molecular biology, its principal aim being the measurement of a specific messenger RNA (mRNA). Before discussing Northern blotting in detail, it is appropriate to consider ...
Please cite this article as: Ying et. al., (2014). RNA Isolation and Northern Blot Analysis, Bio-protocol 4 (6): e1077. DOI: 10.21769/BioProtoc.1077. http://www.bio-protocol.org/e1077 Vol 4, Iss 6, Mar 20, 2014 RNA Isolation and Northern Blot Analysis * Ying Liao, To Sing Fung, Mei Huang, Shouguo Fang, Yanxin Zhong and Dingxiang Liu School of Biological Sciences, Nanyang Technological University, Singapore, Singapore *For correspondence: ...
SCHOLARS The Nigerian Online Publishing Press (http://scholars.ufumes.com) Principles and procedures of the Northern blot technique Francis U Umeoguaju* (2009) Dept of Applied biochemistry, Nnamdi Azikiwe University. Awka. Nigeria. (Review article.) Correspondence to: UF Umeoguaju, talktoumeh@yahoo.ca, +2348067817864 ABSTRACT Northern blot technique is very useful in the analysis of RNA Our present knowledge about the biological events that occurs in molecules. Most of ...
MEDICAL BIOLOGICAL LABORATORIES CO., LTD. 5-3 Sakae 4 chome, Naka-ku, Nagoya 460-0008, Japan TEL: +81-52-238-1901 FAX: +81-52-238-1440 E-mail: info@mbl.co.jp www.mbl.co.jp Protocol for Northern Blotting Northern blotting was performed using DIG Wash and Block Buffer Set (Sigma-Aldrich; code no. 11585762001). For more information, please contact Sigma-Aldrich Co, LLC. Day 1 Electrophoresis and Transfer ↓ Dilute total RNA samples with 2×Loading ...
Johannes Schmid 25.09.1996 RNA-Preparation and Northern Blotting Extraction of RNA....................................................................................................................................... 1 Preparation of total RNA........................................................................................................................ 1 Quantification of the RNA...................................................................................................................... 2 Dot Blot (otional):................................................................................................................................... 2 Electrophoresis in denaturing Agarose-Gels.......................................................................................... 2 Preparation of the gel (1% Agarose)..................................................................................................... 2 Preparation of RNA-samples for electrophoresis............................................................................... 3 Electrophoresis ...................................................................................................................................... 3 Capillary Blot.............................................................................................................................................. 3 Methyleneblue staining of the Blot (optional)..................................................................................... 5 Northern Blot Hybridisation...................................................................................................................... 5 Pre-hybridisation.................................................................................................................................... 5 32P-labelling of ...
DNA Variation VNTR, STR, RFLP, RAPD, AFLP, VNTR and DNA Sequencing Fatchiyah Objectives • Briefly describe VNTR, STR and RFLP • Describe the principle, including the target, for: Southern, Western, Northern and Southwestern blots. • Compare and contrast the 4 blotting methods. • Given a target state the best method for detection. • State 2 clinical applications for detecting VNTRs, STRs and RFLPs. • Given ...
1 TMM,4-06 Northern Blotting (acc. to Maniatis) For work with RNA: Be aware of RNases! Use gloves, wash gloved hands with water, dry. Always use freshly autoclaved not yet opened pipet tips / Eppis! Treat pipets with RNase Erase spray! Preparation of gel and buffers: DEPC-Water (inhibits RNase activity): 0,01% DEPC in Millipore water. Let stand ON, autoclave. Instead autoclaved water may be ...
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