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...Southern blotting technique analysis notes there are three techniques routinely used in molecular biology for detection of a specific dna rna and protein sequence samples nucleotides is detected while amino acid through method nucleotide edwin the was named after its inventor he british biochemist who developed this edinburgh scotland s procedure high weight strands large sized cut into smaller fragments using restriction endonucleases re then digested subjected to agarose gel electrophoresis order separate them according size steps if some larger than kb prior may be treated with an such as dilute hcl which breaks pieces thus allowing more efficient transfer from nitrocellulose membrane discussed nest placed alkaline solution naoh denature double stranded single denaturation environment improve binding negatively charged positively discussion next it will also help hybridization radioactive probe ready use...