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...Content defination of blotting history southern northern western application transfer dna or rna protiens fractinated by gel electrophoresis to a nitrocellulase paper nylon mesh for separation identification and analysis is called blot it carried out allowing liquid seep through electrophoresed nitrocellulose pile filter papers they are very important hybridisation experiments determine the mrna in gels general membrane used tests this technique was first devised ed fragments from sample on steps involved cut with restriction enzyme produce different sizes subjected which results based their size denatured alkali single stranded...