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...Peptidesequencingby secondaryarticle edmandegradation article contents introduction johnbryansmith celltechchiroscienceplc slough uk thebasisofthemethod theroleofpeptidesequencingwithedmanchemistry edmanchemistryisalong establishedapproachtodeterminationoftheprimarystructure or sequence of polypeptides sion seethenextsection andcouldundertakesolid phase bythemiddle the twentieth century it was known that sequencing as well later developments were automated proteinswerecomposedofaminoacids butnothowthese on line phenylthiohydantoin amino acid identication joined together they arranged in blocks by high performance liquid chromatography and similar residues randomly mixed still morethanonereactioncartridgesothatonesequence repeated patterns at about time methods run could automatically be followed another today devised to provide an answer these questions namely manual is rarely done automation has each individual protein its constituent broughtsavingsinresourcesandtimeand withminimiza l...