1 DNA Sequencing (NOTES) DNA sequencing is the process of determining the exact order of nucleotides within a DNA molecule. This method is used to determine the order of the four bases—adenine (A), guanine (G), cytosine (CY), and thymine (T) in a strand of DNA. The advent of rapid DNA sequencing methods has greatly accelerated the biological and medical research. Four nitrogenous bases of DNA DNA structure DNA sequencing has been feasible because of the following developments 1. Availability of restriction endonucleases 2. Development of highly sensitive gel electrophoresis technique, which can separate DNA fragments, differing by only one ...
The principles of DNA Sequencing The process of determining the order of the nucleotide bases along a DNA strand is called sequencing. In 1977, twenty-four years after the discovery of the structure of DNA, two separate methods for sequencing DNA were developed: the chain termination method and the chemical degradation method. Both methods were equally popular to begin with, but, for many reasons, the chain termination method is the method more commonly used today. This method is based on the principle that single-stranded DNA molecules that differ in length by just a single nucleotide can be separated from one another ...
June 2009 KUWAIT MEDICAL JOURNAL 117 Original Article Heat Treatment of Bacteria: A Simple Method of DNA Extraction for Molecular Techniques 1 1 2 3 Ali A Dashti , Mehrez M Jadaon , Abdulsamad M Abdulsamad , Hussein M Dashti 1 Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Health Science Center, Kuwait University, Kuwait 2 Department of Surgery, Amiri Hospital, Kuwait 3 Department of Surgery, Faculty of Medicine, Health Science Center, Kuwait University, Kuwait Kuwait Medical Journal 2009; 41 (2): 117-122 ABSTRACT Objective: To evaluate the efcacy of two simple irradiation for 10 seconds. For comparison, DNA methods involving use ...
DNA Variation VNTR, STR, RFLP, RAPD, AFLP, VNTR and DNA Sequencing Fatchiyah Objectives • Briefly describe VNTR, STR and RFLP • Describe the principle, including the target, for: Southern, Western, Northern and Southwestern blots. • Compare and contrast the 4 blotting methods. • Given a target state the best method for detection. • State 2 clinical applications for detecting VNTRs, STRs and RFLPs. • Given the background information and results of a blot, interpret the results. Introduction • A wide variety of techniques exist in the molecular laboratory. • Knowledge of the targets and appropriate techniques to detect them are ...
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