Chapter 1 – Introduction Textbook: „Introduction to Mammalian Cell Culture“ (D. Ler, U. Glamolija, M. Suljagi) Introduction • Cell Culture – removal of cells from an organism and their subsequent growth in a favorable, artificially controlled environment • Primary Cell Culture – removal from tissue • Can be sub-cultured • Cell line – due to proliferation • Finite – limited life span • Immortal ...
?What is tissue culture In vitro culture (maintain and/or proliferate) of cells, tissues or organs. Types of tissue culture • Cell culture • Primary explant culture • Organ culture 2 Three major categories of tissue culture Cell culture: Adherent monolayer on a solid substrate (various cell types) or suspension in the culture medium (few cell types). Primary explant culture: A fragment of tissue attachment ...
?Why do we need Cell culture • Research • To overcome problems in studying cellular behavior such as: • confounding effects of the surrounding tissues. • variations that might arise in animals under experimental stress. • Reduce animal use. • Commercial or large-scale production. • Production of cell material: vaccine, antibody, hormone. Initiation of culture Animal Plant Animal Plant Tissue Tissue Primary culture Primary ...
Uses Identification Further studies (e.g., Pathogenicity, antiviral sensitivity, research) Limitations Absence of detection system for the agent Inappropriate culture systems Viruses that cannot be cultured A negative viral culture results does not mean that the agent is absent Specimens used to culture viruses Blood specimens Stools, rectal swabs EDTA Urine Heparin Saliva Serum Cerebro-spinal fluid Stool Biopsy Throat swabs Skin (filoviridae) Naso-paryngeal aspirates Organs ...
TISSUE, CELL AND ORGAN CULTURE-1 • Tissue culture Technology is known as the general term to describe the in vitro cultivation of organs, tissues & cells at defined temperature using an incubator, supplemented with a medium containing cell nutrients & growth factors. • Tissue culture : the small tissue fragments are explanted into a suitable medium and encouraged to grow in isolation, to ...
Tissue, cell and organ culture-1 Tissue culture Technology is known as the general term to describe the in vitro cultivation of organs, tissues & cells at defined temperature using an incubator, supplemented with a medium containing cell nutrients & growth factors. Tissue culture : the small tissue fragments are explanted into a suitable medium and encouraged to grow in isolation, to form colonies ...
INTRODUCTION TissueCulture Plant Animal Tissue Culture Tissue Definition: Culture Plant-tissue culture is in-vitro cultivation of plant cell or tissue under aseptic and controlled environment conditions, in liquid or on semisolid well defined nutrient medium for the production of primary and secondary metabolites or to regenerate plant. In other words it is an experimental technique through which a mass of cells (callus) is produced from an ...
Introduction The figure shows a subconfluent (covering ~70% of plastic surface) monolayer (single layer of growing cells) of primary chicken embryo fibroblasts (a cell type that gives rise to connective tissue) under high magnification using phase-contrast microscopy (100X objective). Note the long spindle-shaped morphology of adherent fibroblasts and rounded refractile (dead) cells in the labeled view. Methods Tissue from a 10-day-old embryonated chicken egg was ...
Background and Stage-Setting • I spent 18 yrs at NIEHS doing male reproductive tox, developing methods in primary cell culture, running a lab and overseeing contract studies. • Been at Pfizer for 9 yrs now, in a lab which develops in vitro predictive assays and troubleshoots DART issues. • I am a very strong advocate of and believer in a future for toxicology based increasingly ...
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