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picture1_Tissue Culture Slideshare 77082 | Phase Contrast Microscopy Of Primary Chicken Embryo Fibroblast Cells


 128x       Filetype PPTX       File size 0.80 MB       Source: asm.org


File: Tissue Culture Slideshare 77082 | Phase Contrast Microscopy Of Primary Chicken Embryo Fibroblast Cells
introduction the figure shows a subconfluent covering 70 of plastic surface monolayer single layer of growing cells of primary chicken embryo fibroblasts a cell type that gives rise to connective ...

icon picture PPTX Filetype Power Point PPTX | Posted on 02 Sep 2022 | 3 years ago
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...Introduction the figure shows a subconfluent covering of plastic surface monolayer single layer growing cells primary chicken embryo fibroblasts cell type that gives rise to connective tissue under high magnification using phase contrast microscopy x objective note long spindle shaped morphology adherent and rounded refractile dead in labeled view methods from day old embryonated egg was dissected aseptic technique followed by mincing trypsinizing addition trypsin proteolytic enzyme facilitates breakdown into seeding t culture flasks were grown at oc co dulbecco s minimal essential medium containing fetal calf serum discussion are harvested directly an organism can be for several weeks vitro specialized before undergo senescence especially sensitive chemicals toxins viruses including influenza virus chick kidney eastern equine encephalitis fibroblast often used various research industrial applications avian strains replicate which is important study isolation these investigators have a...

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