Tissue Culture Slideshare 77612 | Mammalian Cell Culture Lab2 Spring 2018

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File: Tissue Culture Slideshare 77612 | Mammalian Cell Culture Lab2 Spring 2018

Chapter 1 – Introduction Textbook: „Introduction to Mammalian Cell Culture“ (D. Ler, U. Glamolija, M. Suljagi) Introduction • Cell Culture – removal of cells from an organism and their ...

Filetype Power Point PPTX | Posted on 03 Sep 2022 | 2 years ago

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...Chapter introduction textbook to mammalian cell culture d ler u glamolija m suljagi removal of cells from an organism and their subsequent growth in a favorable artificially controlled environment primary tissue can be sub cultured line due proliferation finite limited life span immortal transformation continous contains heterogenous population culturing leads generation different purification methods sample dissection rinsing mechanical dissagregation enz digestion trypsin separation techniques density gradient macs facs figure are harvested diluted fresh medium replaced new flask promote further essential for viable state cuture sec transfer one another vessel providing nutrients growing space types lines on the basis number generations doublings slow rate doubling time h continuous transformed under laboratory conditions grow monolayer or suspension rapid strain positive selection either by cloning division potential lose ability proliferate hayflick limit...

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Posted on: 03 Sep 2022 | 2 years ago
Tag: download pptx, mammalian, cell, culture, lab2, spring, 2018,
Filetype: PPTX
File size: 1.81 MB
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