Differences between Conventional and Fluorescent Microscope aThe Conventional a A fluorescence e microscope, uses a microscope uses much higher intensity visible light (400- light source which 700 nanometers) excites a fluorescent species in a sample of to illuminate and interest. This produce a fluorescent species in turn emits a lower magnified image energy light of a of a sample. longer wavelength that produces the magnified ...
Microscopes • Microscopes and microbiology linking advance •The microscope is the microbiologist’s most basic tool . • Microscopes use lenses to magnify object’s images. • There are two types of microscopes: Light microscopes (4 types) Electron microscope (2 types) Light microscope used to examine cells at relatively low magnifications and electron microscope used to lock at cells and cell structure at very high ...
A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study . organic or inorganic substances Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic .radiation The fluorescence microscope was devised in the early part of the twentieth century by August Kohler, Carl Reichert, and Heinrich Lehmann ...
NEW TECHNIQUES IN MICROSCOPY: • Confocal Microscopy (Confocal Scanning Laser Microscope) Light Microscope Electron Microscope Cheap to purchase Expensive to buy .Cheap to operate .Expensive to produce electron beam .Small and portable .Large and requires special rooms .Simple and easy sample preparation .Lengthy and complex sample prep .Material rarely distorted by preparation .Preparation distorts material .Vacuum is not required .Vacuum is required .Natural color of ...
Staining: is a technique used for microscopy to enhance contrast in the microscopic image in biology and medicine to highlight structures in cell populations .or organelles within individual cells .The term stain and dye are not the same A dye is a coloring agent that is used for .general purposes A stain is one that is used for biological .purposes Dyes and Stains Structure Dyes ...
Topics Discussed... 1. INTRODUCTION 2. Hologram & Holography 3. History of holography 4. Photography vs Holography 5. HOLOGRAM- Recording & Reading 6. Usages 7. Types of hologram 8. Holographic Memory 9. Holographic Versatile Discs (HVD) 10.Applications 8/29/22 10:42:19 PM 2 Hologram & Holography A hologram is a flat surface that, under proper illumination, appears to contain a three-dimensional image. Holography is ...
Holography 2 History of Holography • Invented in 1948 by Dennis Gabor for use in electron microscopy, before the invention of the laser • Leith and Upatnieks (1962) applied laser light to holography and introduced an important off-axis technique 3 Conventional vs. Holographic photography • Conventional: –2-d version of a 3-d scene –Photograph lacks depth perception or parallax –Film sensitive only to ...
« One of the most interesting problems that I have ever met with » Incandescen R.W. Wood, 1902 t lamp Spectromete r « I was astounded to find that under certain conditions, the drop from maximum illumination to minimum, a drop certainly of from 10 to 1, Ruled grating occured within a range of wavelengths not greater than the distance between ...
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