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...Chromatography the process or technique of separating molecules components in a mixture solution gas liquid according to differential absorption and elution invented by russian botanist mikhail tsvet is physical separation into its individual used qualitative quantitative analysis biological chemical substances this employs two immiscible mobile phase stationary works as transporter moves definite direction solid absorbs impedes different degrees ion exchange based on charge for almost any kind charged large proteins small nucleotides amino acids preserves analyte column ionic interactions phage buffer ph salt concentration opposite solute ions attracted electrostatic force resin covalently attach anions cations onto it principle relies between...