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...Introduction to chromatography column and hplc pharmaceutical instrumental analysis lina a dahabiyeh basic of is separation technique seprate mixtures into their components in order identify qualitative purify or quantify quantitive the mixture most frequently used analytical pharmaceuticalanalysis aims separate chemical substances its individual so that can be thoroughly analyzed twophases stationary phase stays place liq solid mobilephase flowsthroughcolumnormoveinaplate carriesanalyte liqorgas history russian botanist mikhail tswett coined term describe his experiments separating different colored constituents leaves through khroma colour andgraphic writing classification based on mechanism normal reversed ion exchange affinity shape planer physical state mobile liquid lc gas gc purpose preparative...