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picture1_Sanger Sequencing Slideshare 67000 | 9780367263768 C011


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File: Sanger Sequencing Slideshare 67000 | 9780367263768 C011
Courtesy of CRC Press/Taylor & Francis Group Figure 11.2 Sanger sequencing with fluorescent nucleotides. Rather than using four separate radioactive nucleotides in the sequencing reactions, use of nucleotides ...

icon picture PPTX Filetype Power Point PPTX | Posted on 28 Aug 2022 | 2 years ago

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...Courtesy of crc press taylor francis group figure sanger sequencing with fluorescent nucleotides rather than using four separate radioactive in the reactions use each labeled a different type fluorescence reduced both number top and lanes on polyacrylamide gel middle also enabled automated detection bottom reducing human error reading bands an x ray film as previously see shotgun this methodology applied to whole bacterial genomes involves first randomly dividing genome into fragments that are cloned library order they were is lost sequenced it produces overlapping sequence data joined computationally generate contiguous regions difference between comparative genomics evaluating differences similarities two sequences shows there some one not other blue vice versa red next generation principles most technologies by synthesis established method but modifications dna fragmented technology specific adapter ligated ends adapters used attach solid surface bead or flowcell primers for amplifi...
								

							

						
	
												
				
					
					
					
			
					
				
				
		
	

	
		





	

	
				

						

						
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Posted on: 28 Aug 2022 | 2 years ago
										
										
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												Wan learn