Microbiology Revision Date: December 8, 2017 Determination of Heterotrophic Plate Count (HPC) in Water by Pour Plate, Spread Plate, Membrane Filtration and Enzyme Substrate Test Methods Parameter(s) Heterotrophic Plate Count Analytical Method Pour Plate Spread Plate Membrane Filtration Enzyme Substrate Introduction The heterotrophic plate count (HPC), formerly known as the standard plate count, is a procedure for estimating the number of live culturable g- heterotrophic ...
Technical Specification Sheet Plate Count Agar (Standard Methods) (NCM0010) Intended Use Plate Count Agar (Standard Methods Agar) is used for the enumeration of bacteria in water, wastewater, food, and dairy products in a laboratory setting. Plate Count Agar is not intended for use in the diagnosis of disease or other conditions in humans. This formula conforms to ISO 4833-1&2:2013, ISO 17410:2001, American Public ...
COUNTING BACTERIA Many studies require the quantitative determination of bacterial populations. The two most widely used methods for determining bacterial numbers are the standard, or viable, plate count method andspectrophotometric (turbidimetric) analysis. Although the two methods are somewhat similar in the results they yield, there are distinct differences. For example, the standard plate count method is an indirect measurement of cell density and reveals information ...
1 Microbiological Methods I-A- MESOPHILIC AEROBIC BACTERIA (STANDARD PLATE COUNT OR TOTAL PLATE COUNT) PRINCIPLE Viable bacteria are quantitated by the fractional gram pour plate technique. SCOPE The method is applicable to starches, syrups and sugars and most coproducts of the corn wet milling industry. MEDIA AND REAGENTS 1. Plate Count Agar (PCA). Prepare according to the manufacturer's directions. 2. Butterfield's Phosphate Diluent ...
® SimPlate Total Plate Count Introduction ® SimPlate for Total Plate Count (TPC) method is used for the detection and quantification of the total aerobic ® microorganisms in food. The medium/sample mixture is dispensed into a SimPlate device and incubated for 24-28 h. The total aerobic plate count is determined by counting the fluorescent wells and referring to the ® ® SimPlate Conversion Table. The ...
Microbiology Laboratory (BIOL 3702L) Page 1 of 8 POUR PLATE DETERMINATION OF BACTERIA NUMBERS Principle and Purpose Some microbiological investigations require the determination of the number of microbes present in a culture, solution, etc. There are several means of making this quantitative measurement. In this exercise, students will use two commons methods: the standard plate count and spectrophotometric analysis. The former is based upon the ...
1 Microbiological Methods IV-A- COLIFORM GROUP OF BACTERIA (STANDARD PLATE COUNT METHOD) PRINCIPLE Coliform bacteria are quantitated by the fractional gram pour plate technique (Note 1). SCOPE The method is applicable to starches, syrups and sugars of the corn wet milling industry. SPECIAL APPARATUS Test tubes containing gas collector tubes (Durham Tubes) MEDIA AND REAGENTS 1. Brilliant green lactose bile broth (BGLB), 2% (Note 2) ...
Serial Dilution Protocols | | Created: Friday, 30 September 2005 Author • Jackie Reynolds Information It is a common practice to determine microbial counts for both liquid and solid specimens---suspensions of E. coli in nutrient broth all the way to soil samples and hamburger meat. Most specimens have high enough numbers of microorganisms that the specimen has to be serially diluted to quantitate effectively.The ...
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