microbiology laboratory biol 3702l page 1 of 8 pour plate determination of bacteria numbers principle and purpose some microbiological investigations require the determination of the number of microbes present in ...
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...Microbiology laboratory biol l page of pour plate determination bacteria numbers principle and purpose some microbiological investigations require the number microbes present in a culture solution etc there are several means making this quantitative measurement exercise students will use two commons methods standard count spectrophotometric analysis former is based upon ability viable organisms to grow on medium it not measure non cells contrast method measures turbidity i e density which can be related cell however latter does distinguish between live dead when counts measurements performed conjunction with one another as done curve developed estimate spectrophotometry involves serial dilution sample buffer water or broth media subsequently small volume selected dilutions placed then incubated permit formation single colonies presumably each colony results from rapid growth using size petri dish x mm successful given result better termed forming units cfu term used because cannot coun...