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...Microbiology revision date december determination of heterotrophic plate count hpc in water by pour spread membrane filtration and enzyme substrate test methods parameter s analytical method introduction the formerly known as standard is a procedure for estimating number live culturable g bacteria measuring changes during treatment distribution or swimming pools four prescriptive options are described summary section below choose that best complies with application information licence must be obtained from public health agency canada phac to purchase control organisms required these tests refer website reference simple perform can accommodate volumes sample diluted ranging mls colonies produced relatively small compact less likely encroach on each other than those surface growth however submerged slower growing difficult transfer thermostatically controlled bath essential tempering agar care needed prevent heat shocking when dispensing hot replicating every volume dilution plated analy...