Northern Blotting

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Northern Blotting Transfer the gel O/N 1. Prewet the membrane for 5-10 minutes in SSC 20X. 2. Place the membrane on the Whatman paper. 3. UV Crosslink. 4. Rinse the ...

Filetype PDF | Posted on 17 Sep 2022 | 2 years ago

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...Northern blotting transfer the gel o n prewet membrane for minutes in ssc x place on whatman paper uv crosslink rinse with distilled water dry pre imb hyb temp divided by c length to prehybridize heated dig easy hib ml cm denature probe boiling and cool ice fast dilute of pour off add soln incubate hours washes wash twice sds each at must be warmed temperature high detection washing buffer ul blocking antibody equilibrate color substrate a few dark bcip vial into stop reaction...

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Posted on: 17 Sep 2022 | 2 years ago
Tag: download pdf, northern, blotting,
Filetype: PDF
File size: 0.02 MB
Views: 107x
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