Cell Lines: Types, Nomenclature, Selection and Maintenance The development and various other aspects of primary culture are described above. The term cell line refers to the propagation of culture after the first subculture. In other words, once the primary culture is sub-cultured, it becomes a cell line. A given cell line contains several cell lineages of either similar or distinct phenotypes. It is possible to ...
Animal Cell Culture Rohit Sagar Assistant Professor Deshbandhu college What is cell culture ? • Cell culture is a technique which involves isolation of cells from animal/plant body i.e. from their natural environment (in vivo) and practicing to grow isolated cells in cell specific media in plastic flask or petri dish in a controlled environmental artificial condition (in vitro). • Cell culture means to keep ...
Animal Cell Culture Introduction ACC-is the process of culture animal cells outside the tissue (in vitro) from whichtheywereobtained.Itwillcontinuetogrowifsuppliedwithappropriate conditions and nutrients The culture process allow single cells to act as independent units, much like bacterium or fungus Thecellsarecapableofdividing Animalcellculturewassuccessfullyestablishedin1907 Advantages Consistency and reproducibility of results Toxicological testing procedures are less expensive Reducedchanceofcontamination Disadvantages Cellcharacteristic can change after a period of continuous growth Types of ...
Lec.4 Biotechnology Animal tissue culture Animal tissue culture: is the growth of tissues separate from the animal in vitro (in the laboratory culture media). Cell line Every cell present in the human body is not capable of growing in the laboratory, only a few types of cells can grow in vitro, but they are neither suitable for industrial use nor for scientific purpose, why ...
1 Basic Principles of Cell Culture R. Ian Freshney Centre for Oncology and Applied Pharmacology, Cancer Research UK Beatson Laboratories, Garscube Estate, Bearsden, Glasgow G61 1BD, Scotland, UK, i.freshney@ntlworld.com 1. Introduction ...................................................... 4 2. Types of Cell Culture.............................................. 4 2.1. Primary Explantation Versus Disaggregation .................... 4 2.2. Proliferation Versus Differentiation ............................ 4 2.3. Organotypic Culture ......................................... 7 2.4. Substrates and Matrices ...................................... 9 3 ...
Asian Fisheries Science 22 (2009): 1-6 541 Asian Fisheries Society, Selangor, Malaysia Available online at www.asianfisheriessociety.org Development of a Cell Culture System From Gill Explants of the Grouper, Epinephelus malabaricus (Bloch and Shneider) K. S. SOBHANA*, K. C. GEORGE, G. VENKAT RAVI, GIJO ITTOOP and R. PAULRAJ Marine Biotechnology Division, Central Marine Fisheries Research Institute, P. B. No. 1603, Kochi, India – 682018 ...
Microbiology Laboratory (BIOL 3702L) Page 1 of 6 BASIC CULTURE TECHNIQUE: STREAK PLATE Principle and Purpose The isolation of pure cultures of microorganisms is a technique essential to many types of experiments in microbiology as well as in the identification of potential pathogens. One very common way to isolate bacterial and other microbes is by employing the streak plate technique. In essence, the streak plate ...
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