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...Asian fisheries science society selangor malaysia available online at www asianfisheriessociety org development of a cell culture system from gill explants the grouper epinephelus malabaricus bloch and shneider k s sobhana c george g venkat ravi gijo ittoop r paulraj marine biotechnology division central research institute p b no kochi india abstract was developed successfully malabar tissue samples aseptically excised healthy juveniles e were explanted in leibovitz l medium supplemented with m nacl fetal bovine serum fbs mixture different types cells emerged these observed to spread attach flasks second day onwards confluent monolayers comprising epithelioid as well fibroblast like formed within ten days found grow subcultured by trypsinization seeded into new which produced predominantly subsequent passages introduction vitro systems are necessary for isolation characterization viruses diagnostic reagents testing therapeutics production materials immunological vaccination studies lin...