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...Cell lines types nomenclature selection and maintenance the development various other aspects of primary culture are described above term line refers to propagation after first subculture in words once is sub cultured it becomes a given contains several lineages either similar or distinct phenotypes possible select particular lineage by cloning physical separation some method such derived referred as strain strains do not have infinite life they die divisions finite cells divide only limited number times before their growth rate declines eventually with spans normally i e population doublings extinction actual depends on species differences conditions etc human generally while murine dying continuous few may acquire different morphology get altered capable growing faster resulting an independent progeny from these has unlimited unlike which originated designated transformed immortal tumorigenic for be obtained normal cultures treating them chemical carcinogens infecting oncogenic virus...