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...Microbiology culturemedia iv semestermsczoology midhila mohan assistant professor on contract animal cell and tissue culture media natural artificial the nutrient used for of cells tissues it must be able to support their survival as well growth i e provide nutritional stromal factor are divided in two they naturlal choice medium depends mainly type cultured normal immortalized or transformed objective differentiation production desired protein non primary cultures from healthy require defined quantities proteins factors hormones produce most these but may still need some present serum synthesize fact addition even detrimental such cases like insulin transferrin lipids etc...