Experiment-8: Paper Chromatography for separation of Amino acids Aim: To separate Amino acids by paper chromatography. Principle: Amino acids are the building blocks of peptides and proteins. They possess two ...
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...Experiment paper chromatography for separation of amino acids aim to separate by principle are the building blocks peptides and proteins they possess two functional groups carboxylic acid group gives acidic character provides basic r represents side chain that is different each commonly found in however all have a free primary amine except proline which has cyclic secondary we use properties provided these characterize common provide base nature chains solubilities can be utilized identify their rate migration this aspartame an artificial sweetener its hydrolysis products from certain foods materials solutions aspartic phenylalanine leucine solution equal diet coca cola m hcl ninhydrin spray butanol acetic water solvent mixture aluminum foil whatman no chromatographic x cm ruler polyethylene gloves capillary tubes open on both ends heat lamp or hair dryer drying oven oc wide mouth jar iodine crystals test mm procedure dissolve mg ml tube with bunsen burner using small flame micro boil ...