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...Wr southern blotting restriction and gel electrophoresis of genomic dna digest about g in l reaction volume with u enzymes for h or overnight the warm oven under mineral oil correct temperature use first enzyme incubate then remaining pipette well time pay attention to peculiarities stop by adding probe buffer loading dye sds heat min at c spin briefly prepare agarose according interesting fragment size x tbe ml ethidiumbromid gloves carcinogenic pocket comb mm thick medium sufficient running over lay load samples suitable markers left right different amounts run vh a maximum v is possible take note voltage hours documentation detach piece lower end corner photograph ruler expose only shortly uv light minute wear protective glasses clean disk previously etoh prior viewing picture fluorescent transfer m naoh on bed hybond n sheets filter paper whatman schleicher schuell gb gelgro e cutting edge moisten water ssc handle membrane tweezers construction capillary blots s drawing guarantee t...