chapter 8 column chromatography in column chromatography the stationary phase is a solid adsorbent and the mobile phase is a liquid just like in tlc the big difference between the ...
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...Chapter column chromatography in the stationary phase is a solid adsorbent and mobile liquid just like tlc big difference between two techniques that while solvent moves up through by capillary action down gravity or external pressure employs very small amount of thin layer on plate uses relatively large quantity contained cylindrical generally applied as purification technique it isolates desired compound from mixture an inert constructed metal glass plastic to be analyzed dissolved top eluent passed application air equilibrium established solute adsorbed eluting flowing because different components have interactions with phases they will carried along varying degrees separation achieved individual elutants are collected drips bottom separated into categories depending how flows if allowed flow percolation called forced positive flash method most often used organic chemistry research laboratories term was coined professor w clark still can done silica gel sio alumina al o adsorbents c...