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picture1_Spectroscopy Lec 6   Fluorimetry   Principles  Instrumentation And Applications


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File: Spectroscopy Lec 6 Fluorimetry Principles Instrumentation And Applications
molecular spectroscopy 2 spectroscopy based on emission spectrofluorometry at the end of this lecture you should be familiar with the following fluorescence phosphorescence fluorophore singlet excited state triplet excited state ...

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...Molecular spectroscopy based on emission spectrofluorometry at the end of this lecture you should be familiar with following fluorescence phosphorescence fluorophore singlet excited state triplet quenching inner filter effect quantum yield f fluorometer spectrofluorometer low pressure mercury vapor lamp high xe arc principles fluorometric analysis is extremely sensitive and used widely in many disciplines when a molecule absorbs electromagnetic energy usually lost as heat deactivated via collisional processes some molecules lose only part collisions then electron drops back to ground by emitting photon lower longer wavelength than was absorbed phenomenon called an estimated all fluoresce especially energetic uv radiation absorbing promotes one electrons from or between any two levels same spin cases transformed which its no paired that...

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