CONTENTS CONTENTS Principle Principle Factors effecting fluorescence intensity Factors effecting fluorescence intensity Instrumentation Instrumentation Applications Applications Conclusion Conclusion References. References. FLUORESCENCE FLUORESCENCE It is a phenomenon of emission of radiation when the molecules are exited by radiation at certain wavelength. FLUORIMETRY:- It is measurement FLUORIMETRY:- It is measurement of fluorescence intensity at a of fluorescence intensity at a particular wavelength wit the help of a particular wavelength wit the help of a filter fluorimeter or a filter fluorimeter or a spectrofluorimeter. spectrofluorimeter. PRINCIPLE:- Molecule contains electrons, PRINCIPLE:- Molecule contains electrons, electrons and non bonding (n) electron. electrons and non bonding ...

 

Fluorimetry Emission Flame photomete r Spectrosco py NMR Absorption IR UV 2 Electromagnetic Spectrum Frequency, n in Hz ~1019 ~1017 ~1015 ~1013 ~1010 ~105 Wavelength, l ~.0001 nm ~0.01 nm 10 nm 1000 nm 0.01 cm 100 m Energy (kcal/mol) > 300 300-30 300-30 ~10-4 ~10-6 g-rays X-rays UV IR Microwave Radio nuclear core electronic molecular molecular Nuclear Magnetic excitation electron excitation vibration rotation Resonance NMR (PET) excitation (p to p*) (MRI) (X-ray cryst.) Visible 3 4 CONTENTS 1. INTRODUCTION 2. THEORY-MOLECULAR VIBRATION 3. INSTRUMENTATION 4. Infrared Group Analysis 5 1. Introduction IR spectroscopy is ...