CONTENTS CONTENTS Principle Principle Factors effecting fluorescence intensity Factors effecting fluorescence intensity Instrumentation Instrumentation Applications Applications Conclusion Conclusion References. References. FLUORESCENCE FLUORESCENCE It is a phenomenon of emission of radiation when the molecules are exited by radiation at certain wavelength. FLUORIMETRY:- It is measurement FLUORIMETRY:- It is measurement of fluorescence intensity at a of fluorescence intensity at a particular wavelength wit the help of a particular wavelength wit the help of a filter fluorimeter or a filter fluorimeter or a spectrofluorimeter. spectrofluorimeter. PRINCIPLE:- Molecule contains electrons, PRINCIPLE:- Molecule contains electrons, electrons and non bonding (n) electron. electrons and non bonding ...
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