Introduction Plants can be propagated by sexual or asexual means. Clonal propagation refers to the process of asexual reproduction by multiplication of genetically identical copies of individual plants, where the term clone is used to represent a plant population derived from a single individual by asexual reproduction. Invitro clonal propagation through tissue culture is referred to as micropropagation. Micropropagation is the practice of rapidly multiplying stock plant material to produce large number of progeny plants, using modern plant tissue culture ...
Application of plant tissue culture Plant tissue culture technology has been used in almost all the field of biosciences. Its applications include 1. Production of phytopharmaceuticals and secondary metabolites. a) Biotransformation (Biochemical Conversion) b) Plant cell immobilization c) Genetic transformation (Transgenic plant) d) Elicitors 2. Micropropagation (Clonal Propagation) 3. Synthetic seed 4. Protoplast culture and somatic hybridization 5. Hairy root culture 6. Cryopreservation 7. Tracing the biosynthetic pathways of secondary metabolites 8. Generation novel compound from plant 9. Respiration, organ ...
Biotechnology in Agriculture There are many important applications of biotechnology that have made a trem endous impact on agricultural productivit y. Conventional plant breeding Tissue culture & micropropagation Molecular breeding or marker assisted selection Genetic engineering & GM crops The ‘Omics’ - Genomics, Proteomics, Metabolomics Plant disease diagnostics Microbial fermentation. Biotechnology in Agriculture GE of animals GE of animals GE of plants GE of plants GE to improve GE to develop GE to improve GE to develop ...
Learning Objectives Understand what clones are. Understand the advantages and disadvantages of natural plant clones. Understand what natural vegetative propagation in plants is. Understand what artificial propagation is. Understand the process of micropropagation. Clones Clone: Genes, cells or whole organisms that carry identical genetic material because they are derived from the same original DNA. Examples: Identical twins (when zygote splits). Plants producing runners. Binary Fission in bacteria. Cloning is a natural process for growth/reproduction, but can also be artificial. Advantages ...
Plant Tissue Culture The culture and maintenance of plant cells and The culture and maintenance of plant cells and organs organs The culture of plant seeds, organs, tissues, cells, or protoplasts on nutrient media under sterile conditions The growth and development of plant seeds, organs, tissues, cells or protoplasts on nutrient media under sterile (axenic) conditions The in vitro, aseptic plant culture for any purpose including genetic transformation and other plant breeding objectives, secondary product production, pathogen elimination or for ...
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