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picture1_Radioimmunoassay Slideshare 77668 | 25011 Item Download 2022-09-03 07-00-10


picture2_Radioimmunoassay Slideshare 77668 | 25011 Item Download 2022-09-03 07-00-10 picture3_Radioimmunoassay Slideshare 77668 | 25011 Item Download 2022-09-03 07-00-10

 80x       Filetype PPT       File size 0.07 MB       Source: www.pitt.edu


File: Radioimmunoassay Slideshare 77668 | 25011 Item Download 2022-09-03 07-00-10
Principle of Radioimmunoassay • Principle: Uses an immune reaction [Antigen – Antibody reaction] to estimate a ligand Ag + Ag* + Ab AgAb + Ag*Ab + Ag + Ab* &ndash ...

icon picture PPT Filetype Power Point PPT | Posted on 03 Sep 2022 | 2 years ago

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...Principle of radioimmunoassay uses an immune reaction to estimate a ligand ag ab agab unbound and washed out radioactivity bound residue measured conc is inversely related advantages disadvantages ria highly specific reactions are high sensitivity sensitive radiation hazards radiolabelled reagents requires specially trained persons labs require special license handle radioactive material arrangements for requisition storage waste disposal requirements preparation characterisation the antigen radiolabelling antibody development assay system antigens prepared by synthesis molecule isolation from natural sources h c i used as tags tagged tagging should not affect antigenic specificity activity injected intradermally into rabbits or guinea pigs production antibodies recovered serum some ligands hormones steroids drugs haptens eg gastrin morphine conjugated albumin...
								

							

						
	
												
				
					
					
					
			
					
				
				
		
	

	
		





	

	
				

						

						
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										Category:  Uncategorized / Uncategorized 
										
Posted on: 03 Sep 2022 | 2 years ago
										
										
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Filetype: PPT
										
File size: 0.07 MB
										
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												Wan learn