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...Radioimmunoassay ria is a very sensitive in vitro assay technique used to measure concentrations of antigens for example hormone levels the blood by use antibodies extremely and specific requiring specialized equipment history was introduced berson yalow as an concentration insulin plasma it represented first time that could be detected has revolutionized research clinical practice many areas e g banking diagnosis allergies endocrinology principle based on ability unlabelled form substance inhibit competitively binding radioactively labelled i labeled antigen ag serum ab serial dilutions one starts with combination corresponding measured antibody then quantity unlabeled or cold added mixture competes radioactive displaces proportional amount unbound separated away centrifugation radioactivity remaining this process continually repeated using progressively greater line graph demonstrating relationship between constructed creates standard curve competitive biological sample tested compar...