background gel filtration is liquid chromatography which separates molecules according to their size also known as size exclusion chromatography sec gel permeation chromatography molecular sieve chromatography used in separation of ...
Filetype Power Point PPTX | Posted on 28 Aug 2022 | 4 years ago
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...Background gel filtration is liquid chromatography which separates molecules according to their size also known as exclusion sec permeation molecular sieve used in separation of macromolecules such proteins peptides nucleic acids and carbohydrates stationary phase beads contain pores specific usually polysaccharides dextran or other polar polymers formulated into small theses varying degrees cross linking the polysaccharide within bead allowing smaller pass through while larger are excluded principle sample a column packed with swollen separating occurs weight large that than largest pore can t penetrate move around from quickly elute first retained because different depends on eluted order decreasing so works opposite...